Research Escalators
HCAR provides facilitated access to research resources and expertise, especially in the areas of research discovery, development and commercialization.
Amenities include:
Click below to see the equipment and core facilities that are available onsite at HCAR to resident organizations.
- High-throughput RT-PCR
- Applied Biosystems 7300 Real Time PCR system
- Applied Biosystems 7900 HT Fast Real Time PCR system
- Eppendorf Mastercycler thermocycler
- Eppenorf Thermomixer R
- Laser capture microdissection
- Microlaser Technologies/Zeiss PALM-LCM system/Axiovert microscope
- Bioinformatic analysis
- 2-D gel electrophoresis
- Amersham Ettan IPGphor3 and Ettan DALT12
- Amersham Ettan SpotPicker
- NextGen Biosciences A2DEoptimizer gel caster
- Whole genome gene expression and SNP association
- Axon Instruments GenePix 4000B
- Amersham Bioscience Typhoon 9410 Fluorescence imaging system
- UV/Vis plate readers
- Agilent HP2100 Bioanalyzer
- Nanodrop ND-1000 spectrophotometer
- Molecular Devices Spectromax M2 spectrophotometer
- Illumina Bead Station
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- Super speed and high speed ultracentrifuges
- Beckman J2-MI and Avanti J-E
- Rotors JA-14 (2); JA-17; JA-10
- Beckman L8-M Ultracentrifuge (2)
- Rotors SW-41 (2); SW-50.1 Ti; 75 Ti; 70 Ti; 50 Ti
- Shaking Incubators
- Barnstead Max Q5000 Refrigerated
- Barnstead Max Q5000
- Tabletop Ultracentrifuge
- Beckman Optima MAX with TLA100 rotor Room 3102 (Jong Yun x1508)
- Cold room with FPLC protein purification workstation
- AKTA FPLC system
- 2-D gel electrophoresis
- Amersham Ettan IPGphor II and Ettan DALT12
- Cell culture facilities with hypoxic incubator capability
- Each laboratory maintains their own cell culture facilities.
- Each pod has centralized CO2 delivery systems.
- Hypoxic Incubator
- Gel documentation workstation
- Fuji Intelligent Darkbox II w/ cooled CCD camera
- UVP gel documentation stations (2)
- Kodak film processor
- Barrier Animal Facility
- Liquid scintillation counters (2)
- Beckman LS6000; LS6500
- Fluorescence Microscopy
- fluorescent microscopes
- Nanodrop small volume spectrophotometer ND-1000
- High-end mass spectrometry
- NMR (500-600 MHz)
- Confocal microscopy
- Electron Microscopy
- LC/MS/MS
- Flow cytometry and cell sorting
- Transgenic and KO mouse
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Click below to see the core facilities and shared services available to resident organizations.
- Computer & Sequence Analysis / Bioinformatics – computers, programs, and consultations
- Molecular Genetics/DNA Sequencing and SNPlex analyses
- Organic Synthesis Core
- Macromolecular Synthesis Core (Oligo and Peptide Synthesis (normal, modified, and heavy isotope), protein/peptide Edman Sequencing)
- Microscopy & Histology (Multi-Channel Fluorescence Microscopy, Confocal Microscopy, Spinning Disk Confocal Microscopy, Deconvolution Microscopy, Electron Microscopy)
- Mass Spectrometry (Proteomics: iTRAQ and DIGE gel global quantitation, ID of Co-immunoprecipitated proteins, Post-translational modifications, Peptide MRM validation. Small Molecules: Lipidomics, MRM methods development for small molecule identification and quantification)
- Functional Genomics Core Lab – (Gene Expression Analysis: Microarrays with Affymetrix, Agilent, and Illumina platforms; qRT-PCR and SNP detection: TaqMan platform; Laser-Capture Microdissection, high-throughput robotic sample handling)
- Cell Science & Flow Cytometry Core Lab (FACS analysis and Flow Sorting, six instruments)
- Animals-Transgenic Animal production and breeding, Phenotyping, Re-derivation services, Imaging (Bioluminescent Imaging Services, Micro CT imaging services, MRI & MRS for small animals)
- MRI, MRS, and fMRI imaging of human subjects and animals
- Wyatt Light-Scattering detection for analysis of macromolecule size and aggregation state.
- X-Ray Crystallography Facility
- Solution Phase NMR Facility (500 and 600 Hz instruments with cryoprobe)
- Tissue and Tumor Bank
- Viral Vector Core
- Biostatistics Core
- Cancer Informatics Core (emerging)
- Access to Penn State researchers/mentors
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